LOCUS       pET28a-PheRS            8632 bp    DNA     circular SYN 22-JUL-2025
DEFINITION  Nucleus PURE: pET28a-PheRS in pET28a protein expression plasmid. See
            https://nucleus.bnext.bio/ for more details..
ACCESSION   pET28a-PheRS
VERSION     pET28a-PheRS
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     rep_origin      77..665
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        77..105
                     /label="RNAII promoter"
                     /note="Color: #ffffff NCBI Feature Key: promoter NCBI Join
                     Type: join Transferred From: RNAII_promoter Transferred
                     Similarity: 100.00%"
     misc_RNA        113..665
                     /label="RNAII transcript (complementary strand)"
                     /note="RNAII transcript (complementary strand)"
     misc_RNA        complement(116..223)
                     /label="RNAI transcript"
                     /note="RNAI transcript"
     promoter        complement(230..259)
                     /label="RNA I promoter"
                     /note="Color: #ffffff NCBI Feature Key: promoter NCBI Join
                     Type: join Transferred From: RNA_I_promoter Transferred
                     Similarity: 100.00%"
     terminator      669..712
                     /label="rop/bom terminator"
     misc_binding    752..760
                     /label="dnaA binding site"
                     /bound_moiety="dnaA"
     misc_feature    785..848
                     /label="L-strand Y effector site"
                     /note="L-strand Y effector site"
     misc_feature    851..990
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     misc_feature    1031..1187
                     /label="H-strand Y effector site"
                     /note="H-strand Y effector site"
     CDS             complement(1092..1283)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein"
                     /label="rop"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     RBS             1284..1293
     misc_feature    complement(1359..1377)
                     /label="ROP calculated promoter"
     CDS             complement(2092..3174)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label="lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(3175..3252)
                     /label="lacI promoter"
                     /note="Color: #ffffff NCBI Feature Key: promoter
                     Transferred From: lacI_promoter Transferred Similarity:
                     100.00%"
     promoter        3561..3579
                     /label="T7_promoter"
     protein_bind    3580..3604
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3613..3645
                     /label="UTR1"
                     /note="note: UTR1 NCBI Feature Key: misc_feature Color:
                     #cc0000 modified by: Andrey Transferred From: U4m - UTR1
                     Transferred Similarity: 100.00%"
     CDS             3649..3651
                     /codon_start=1
                     /label="Start"
                     /translation="M"
     CDS             3652..3669
                     /codon_start=1
                     /label="His6"
                     /translation="HHHHHH"
     CDS             3670..3684
                     /codon_start=1
                     /label="Linker"
                     /translation="GGGGS"
     CDS             3685..4665
                     /codon_start=1
                     /label="pheS"
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTTL
                     RELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIENG
                     GLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWFDTT
                     RLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNISFTN
                     LKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVHPNVLR
                     NVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     CDS             4683..7067
                     /codon_start=1
                     /label="pheT"
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHGV
                     VVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGDFK
                     IKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEISVT
                     PNRADCLGIIGVARDVAVLNQLPLVQPEIVPVGATIDDTLPITVEAPEACPRYLGRVVK
                     GINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGIVVRMA
                     KEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLECAFFSP
                     LSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVIDITNEAT
                     LPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPSWRFDMEIE
                     EDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQEVITYSFVD
                     PKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNRVRIFESGLRF
                     VPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESVLDLTGKLNEVE
                     FRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVFELEWNKLADRVV
                     PQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNLFDVYRGKGVAEGY
                     KSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     terminator      7141..7188
                     /label="T7 terminator"
                     /note="Color: #ffffff NCBI Feature Key: terminator
                     Transferred From: T7_terminator Transferred Similarity:
                     100.00%"
     rep_origin      7225..7680
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(7772..8587)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label="KanR"
                     /note="confers resistance to kanamycin"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      901 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
      961 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     1021 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg
     1081 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     1141 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     1201 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     1261 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     1321 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     1381 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     1441 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     1501 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     1561 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc
     1621 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     1681 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     1741 cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     1801 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     1861 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     1921 gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata
     1981 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     2041 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
     2101 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     2161 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg agacgggcaa
     2221 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     2281 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     2341 gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     2401 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     2461 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     2521 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gccagccagc
     2581 cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga tttgctggtg
     2641 acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg agaaaataat
     2701 actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat tagtgcaggc
     2761 agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca gcccactgac
     2821 gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc ttcgttctac
     2881 catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg ccgcgacaat
     2941 ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca acgactgttt
     3001 gcccgccagt tgttgtgcca cgcggttggg aatgtaattc agctccgcca tcgccgcttc
     3061 cactttttcc cgcgttttcg cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt
     3121 ctgataagag acaccggcat actctgcgac atcgtataac gttactggtt tcacattcac
     3181 caccctgaat tgactctctt ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca
     3241 ttcgatggtg tccgggatct cgacgctctc ccttatgcga ctcctgcatt aggaagcagc
     3301 ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga
     3361 tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc
     3421 tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc
     3481 cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg
     3541 agatctcgat cccgcgaaat taatacgact cactataggg gaattgtgag cggataacaa
     3601 ttcccctcta gaaataattt tgtttaactt taagaaggag atataccaat gcaccatcac
     3661 caccatcatg gaggcggtgg atctatgagc catttagcgg agctggtcgc gagtgctaag
     3721 gcagctatct cacaggcgtc ggatgtcgcc gcgcttgata atgttcgcgt cgaatatctg
     3781 ggcaaaaaag gccatctgac cttgcagatg accacattgc gcgaactgcc acccgaagaa
     3841 cgtcccgccg cgggtgccgt aatcaatgaa gccaaagaac aggtacagca ggctttaaat
     3901 gctcgcaagg ccgagttaga aagcgcagcg ctgaacgctc gccttgctgc tgagacaatt
     3961 gatgttagcc tgcccggtcg ccgcattgag aatggcggtt tacatccggt tactcgcacg
     4021 atcgaccgca ttgaatcttt tttcggcgaa ctgggtttca ccgttgcaac tggccctgaa
     4081 atcgaagatg actatcataa ttttgatgcg cttaacatcc ctggtcacca tccggcacgg
     4141 gccgaccacg acacgttctg gtttgatacg acgcgcctgt tacgtaccca gactagtggg
     4201 gttcagattc gcaccatgaa ggcacaacag ccgccgattc gcatcattgc tccagggcgt
     4261 gtgtatcgca acgattacga tcaaactcac acgccgatgt tccatcagat ggaaggcctg
     4321 attgtggata cgaatattag ctttacaaat cttaaaggta ccctgcacga ttttttgcgc
     4381 aatttctttg aggaagatct gcagatccgc tttcgcccct cgtattttcc gtttaccgaa
     4441 cctagtgcgg aagtagacgt gatgggtaaa aacggcaagt ggttagaagt actgggatgt
     4501 ggcatggttc acccgaacgt cctgcgtaat gtcggcatcg accctgaagt gtattccggc
     4561 tttgcatttg gcatgggaat ggagcgtttg accatgttac gttatggcgt gaccgacttg
     4621 cgttcctttt ttgaaaatga tttgcgcttc ctgaaacaat ttaagtgagg caggaataga
     4681 ttatgaaatt ctccgaactt tggctgcgtg aatgggttaa tccggcgatt gattctgatg
     4741 cgcttgctaa tcagattacc atggccggtc tggaagttga cggcgtcgaa cctgtagctg
     4801 gtagctttca tggtgttgtt gtgggcgagg tagtcgaatg cgcacagcat ccgaacgctg
     4861 ataaactgcg cgttactaaa gttaatgttg ggggggatcg cttactggat atcgtctgcg
     4921 gcgcaccgaa ctgtcgtcaa ggcctgcggg ttgcagtcgc gacaatcgga gccgtactgc
     4981 ccggggattt taaaattaaa gcagcaaaat tacgtggtga acctagcgaa ggtatgttgt
     5041 gttcgttcag tgaactgggt atcagcgacg accattcggg cattatcgag ctgccagcgg
     5101 acgccccgat tggcacggac atccgggagt acctgaaact ggacgacaac accattgaaa
     5161 tttcggttac cccgaatcgt gccgattgcc tgggcattat tggtgtggca cgtgatgtcg
     5221 cggtgcttaa tcagctgccg ctggtacaac cggaaatcgt accagtgggc gcaaccattg
     5281 atgacacctt accgatcacg gttgaggcac cggaagcatg tcctcgttat ttaggacggg
     5341 tagttaaagg gattaacgtg aaagccccca cgccattatg gatgaaagag aagctgcgtc
     5401 ggtgcgggat tcgtagtatt gatgcagtag tggacgtaac aaactatgta ttgctggagc
     5461 tggggcagcc gatgcatgct tttgacaaag atcgtattga gggtggaatt gtggtccgta
     5521 tggcgaagga gggtgaaacc ttagtgttat tagacggtac ggaagctaaa ttaaatgccg
     5581 atacattggt gattgccgat cataataagg cgttagctat gggaggcatc tttggtggag
     5641 aacatagtgg cgttaacgac gagacacaga acgtattgct ggaatgcgct ttttttagcc
     5701 ctctgtcgat caccggccgc gcgcgccgtc atggactgca taccgatgct agtcaccgtt
     5761 acgaacgcgg tgtcgatccg gccctgcagc acaaggcaat ggagcgcgca acccgcctgc
     5821 ttatcgatat ttgtgggggt gaggctggcc cagtgatcga tatcactaac gaagccaccc
     5881 ttccaaaacg cgcgacgatt accctgcgcc ggtcgaagct ggatcggtta attggacatc
     5941 atattgcaga tgaacaggtt acagacattc tgcgccgtct gggttgcgag gtcacagagg
     6001 gtaaagatga atggcaggct gtggccccta gctggcgttt tgatatggaa attgaagagg
     6061 accttgtaga ggaagtggcc cgcgtctacg gctataataa catcccggat gaaccggtgc
     6121 aggcgagtct gattatgggg actcatcgtg aagcggatct tagcctgaaa cgcgtaaaga
     6181 ctctgctgaa cgacaaaggc taccaggaag tgattaccta ttcattcgtt gatccgaaag
     6241 tccaacaaat gatccacccg ggtgtagaag ctctgttact gcctagcccg atttccgtgg
     6301 aaatgagtgc catgcgctta tcactgtgga cgggtttgtt agcgacggtt gtctataacc
     6361 agaatcggca gcagaaccgc gtccgtatct ttgagtctgg cctgcgtttt gttcctgata
     6421 ctcaggctcc gctgggcatt cgccaagacc tgatgctggc aggcgtcatt tgtggcaacc
     6481 gttatgagga acactggaat ctggcgaagg aaaccgtaga cttctatgac ttaaagggcg
     6541 acctggaatc cgtactggac ctgacaggca aactgaatga ggtcgagttt cgcgctgaag
     6601 ccaacccggc gcttcatccg ggccagagcg ccgctattta ccttaaaggc gaacgtattg
     6661 gtttcgtagg tgttgtacat ccggaattgg aacgcaaatt agatctgaat ggtcgcactc
     6721 tggtctttga actggaatgg aacaaactgg ctgaccgcgt tgttccccag gctcgcgaaa
     6781 tctcccgttt cccagctaat cgtcgtgata tcgcggttgt agtagccgaa aatgttccag
     6841 cggcagatat cttatcagag tgcaaaaaag taggcgtcaa tcaagtggtt ggtgtgaatt
     6901 tatttgacgt gtatcgtggg aaaggcgtgg ccgaaggtta taagagcttg gccatcagct
     6961 tgatcctgca ggatacttca cgtactttag aagaagaaga gattgcagcc acggttgcga
     7021 aatgtgttga agccttgaaa gaacgttttc aggcctcatt acgtgattga agagcttgat
     7081 ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa
     7141 ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga
     7201 actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7261 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7321 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7381 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7441 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7501 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7561 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7621 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     7681 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     7741 ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa
     7801 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     7861 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     7921 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     7981 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagtttat
     8041 gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg
     8101 catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc
     8161 tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg
     8221 catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc
     8281 cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg
     8341 tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat
     8401 tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca
     8461 atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata
     8521 aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc cgttgaatat
     8581 ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt gt
//
