CDK

	Protocol		PURE Timeseries
Experiment File Name:	20251107-cytation3-pure-timecourse-gfp-EXPERIMENT.xpt		
Experiment File Path:	C:\Users\bnext\Documents\Biotek\experiments\20251107-cytation3-pure-timecourse-gfp-EXPERIMENT.xpt		
Protocol File Name:	cytation3-pure-timecourse-gfp		
Protocol File Path:	C:\Users\bnext\Documents\Biotek\protocols\cytation3_protocols\cytation3-pure-timecourse-gfp.prt		
Reading Date/Time:	11/7/2025 12:25:23 PM		
Plate ID:			
Barcode:			
Reader Serial Number:	130723A		
Plate Comment:			

Procedure Summary

Plate Type	Greiner 384 SV NoBind AutoMap
Well Selection	Runtime
Eject plate on completion	
Set Temperature	Temperature:  Setpoint 37 C
Start Kinetic	Start Kinetic [Run 6:00:00, Interval 0:05:00]
Read	Read:  GFP-F-G35 (F) 485/20,528/20
Read	Read:  GFP-M-G100 (F) 485,528
End Kinetic	End Kinetic
Set Temperature	Temperature:  Off

PURE:GFP-F-G35

Time	T	A1	A2	A3	A4	A5	A6	A7	A8	A9	A10	A11	A12	A13	A14	A15	A16	A17	A18	A19	A20	A21	A22	A23	A24	B1	B2	B3	B4	B5	B6	B7	B8	B9	B10	B11	B12	B13	B14	B15	B16	B17	B18	B19	B20	B21	B22	B23	B24	C1	C2	C3	C4	C5	C6	C7	C8	C9	C10	C11	C12	C13	C14	C15	C16	C17	C18	C19	C20	C21	C22	C23	C24	D1	D2	D3	D4	D5	D6	D7	D8	D9	D10	D11	D12	D13	D14	D15	D16	D17	D18	D19	D20	D21	D22	D23	D24	E1	E2	E3	E4	E5	E6	E7	E8	E9	E10	E11	E12	E13	E14	E15	E16	E17	E18	E19	E20	E21	E22	E23	E24	F1	F2	F3	F4	F5	F6	F7	F8	F9	F10	F11	F12	F13	F14	F15	F16	F17	F18	F19	F20	F21	F22	F23	F24	G1	G2	G3	G4	G5	G6	G7	G8	G9	G10	G11	G12	G13	G14	G15	G16	G17	G18	G19	G20	G21	G22	G23	G24	H1	H2	H3	H4	H5	H6	H7	H8	H9	H10	H11	H12	H13	H14	H15	H16	H17	H18	H19	H20	H21	H22	H23	H24	I1	I2	I3	I4	I5	I6	I7	I8	I9	I10	I11	I12	I13	I14	I15	I16	I17	I18	I19	I20	I21	I22	I23	I24	J1	J2	J3	J4	J5	J6	J7	J8	J9	J10	J11	J12	J13	J14	J15	J16	J17	J18	J19	J20	J21	J22	J23	J24	K1	K2	K3	K4	K5	K6	K7	K8	K9	K10	K11	K12	K13	K14	K15	K16	K17	K18	K19	K20	K21	K22	K23	K24	L1	L2	L3	L4	L5	L6	L7	L8	L9	L10	L11	L12	L13	L14	L15	L16	L17	L18	L19	L20	L21	L22	L23	L24	M1	M2	M3	M4	M5	M6	M7	M8	M9	M10	M11	M12	M13	M14	M15	M16	M17	M18	M19	M20	M21	M22	M23	M24	N1	N2	N3	N4	N5	N6	N7	N8	N9	N10	N11	N12	N13	N14	N15	N16	N17	N18	N19	N20	N21	N22	N23	N24	O1	O2	O3	O4	O5	O6	O7	O8	O9	O10	O11	O12	O13	O14	O15	O16	O17	O18	O19	O20	O21	O22	O23	O24	P1	P2	P3	P4	P5	P6	P7	P8	P9	P10	P11	P12	P13	P14	P15	P16	P17	P18	P19	P20	P21	P22	P23	P24
0:00:00	24.4																																		119	12	118	29	136																				12	12	12	11	14																				148	14	110	12	121																				11	14	12	11	12																				117	12	120	12	112																				11	12	12	15	12																				119	12	115	12	118																				12	15	14	12	18																				98	12	128	12	123																				11	12	19	17	14																				39	12	40	13	40																				12	12	11	12	12																				11	13	12	16	12																				12	28	12	17	27																																		
0:05:00	29.6																																		193	12	192	29	220																				12	12	12	12	14																				231	14	196	12	221																				11	15	12	12	12																				144	12	145	12	133																				12	12	12	15	12																				123	11	119	12	121																				12	22	15	12	18																				101	12	131	12	126																				11	12	19	17	14																				42	12	42	13	42																				12	12	12	12	12																				12	13	12	16	12																				12	29	13	18	27																																		
0:10:00	33.4																																		639	12	640	29	730																				12	12	12	12	14																				797	14	767	12	895																				12	14	12	12	12																				533	12	526	12	483																				12	12	13	15	12																				258	11	230	12	218																				12	21	14	12	18																				135	12	158	13	145																				12	12	20	18	14																				102	12	103	13	106																				12	12	12	12	12																				12	13	13	16	12																				12	30	12	18	27																																		
0:15:00	36.2																																		2085	12	2131	28	2410																				12	12	12	11	14																				2911	15	2902	12	3405																				12	14	12	11	11																				2392	12	2396	11	2221																				12	12	12	15	12																				1417	11	1278	12	1231																				12	21	14	12	18																				714	12	721	13	641																				12	12	20	18	14																				412	12	411	13	426																				12	11	12	12	12																				12	13	12	17	12																				12	32	12	18	27																																		
0:20:00	37.0																																		4852	12	5017	28	5738																				12	12	12	12	14																				7563	15	7677	12	9019																				12	14	12	12	12																				7114	12	7215	12	6682																				12	12	12	15	12																				4914	12	4561	12	4466																				12	21	14	11	16																				2973	12	3177	13	3028																				11	11	20	18	13																				1296	12	1296	13	1341																				12	12	12	12	12																				12	13	12	17	12																				12	33	12	18	27																																		
0:25:00	37.0																																		8154	12	8570	29	9855																				12	12	12	12	14																				13617	14	14238	12	16818																				12	14	12	12	12																				14593	12	14875	12	13751																				12	12	12	15	13																				10698	11	10086	12	9894																				12	20	14	12	12																				7023	12	7636	12	7479																				12	12	20	18	13																				3073	12	3086	13	3194																				12	12	12	12	12																				11	13	12	16	12																				12	33	12	17	27																																		
0:30:00	37.0																																		10987	11	11672	28	13575																				12	12	12	12	15																				18915	15	20453	12	24482																				12	15	12	12	12																				22848	12	23467	12	21737																				12	12	13	15	13																				17504	12	16609	12	16307																				12	20	14	12	12																				11920	11	12972	13	12826																				12	12	20	18	14																				5743	12	5799	13	6032																				12	12	12	12	12																				12	13	12	17	12																				12	33	12	18	27																																		
0:35:00	37.0																																		13110	12	13975	29	16423																				12	12	13	12	15																				22938	15	25251	12	30562																				13	15	12	12	13																				30415	12	31366	12	29121																				13	12	13	15	13																				24241	12	23105	12	22586																				12	21	15	12	12																				16886	12	18389	13	18133																				12	11	20	18	14																				9076	12	9147	13	9467																				12	12	12	12	12																				12	13	13	16	12																				12	34	13	18	27																																		
0:40:00	37.0																																		14603	12	15667	29	18381																				12	12	13	12	15																				25852	14	29019	12	35145																				12	15	13	11	12																				36481	12	37724	12	35029																				12	12	13	15	13																				30152	12	28803	12	28160																				12	20	15	12	12																				21462	12	23264	13	23009																				12	12	20	18	15																				12732	13	12818	13	13258																				12	12	12	12	12																				12	13	12	17	12																				12	34	12	18	27																																		
0:45:00	37.0																																		15726	12	16924	29	19858																				13	12	13	12	15																				28022	14	31872	12	38741																				13	14	13	12	13																				41508	12	42752	11	39889																				13	12	13	15	14																				35128	12	33616	12	32939																				12	20	15	12	12																				25578	11	27563	13	27247																				12	12	20	18	15																				16450	12	16561	13	17034																				12	12	12	12	12																				12	13	12	16	12																				12	34	12	18	27																																		
0:50:00	37.0																																		16461	12	17788	29	20935																				12	12	13	12	15																				29652	14	33988	11	41597																				13	15	13	12	13																				45415	12	46837	12	43886																				13	12	14	15	14																				39003	11	37516	12	36795																				13	20	15	12	13																				28924	12	31180	13	30808																				12	12	20	18	15																				20098	12	20188	13	20749																				12	12	12	13	12																				12	13	12	17	12																				12	33	12	18	27																																		
0:55:00	37.0																																		17024	12	18378	29	21645																				13	12	13	12	15																				30809	15	35653	12	43832																				13	15	13	12	13																				48664	12	50214	12	47164																				12	11	14	15	14																				42361	12	40739	12	40027																				13	21	15	12	13																				31835	12	34152	13	33798																				12	12	20	18	15																				23573	12	23637	13	24277																				13	12	12	12	11																				12	13	12	17	12																				11	34	13	17	27																																		
1:00:00	37.0																																		17479	11	18900	29	22280																				13	12	13	12	15																				31705	14	36776	12	45308																				13	15	13	12	13																				51302	12	52873	11	49786																				13	12	14	15	14																				45035	12	43611	12	42608																				13	21	15	12	12																				34185	12	36796	13	36274																				13	12	20	18	15																				26749	13	26865	12	27620																				12	12	12	12	12																				12	13	12	16	12																				12	33	12	18	27																																		
1:05:00	36.9																																		17742	12	19221	29	22672																				13	12	13	12	15																				32525	15	37818	12	46865																				13	14	14	12	13																				53708	12	55110	12	52053																				13	12	14	15	14																				47265	12	45787	12	44935																				13	20	15	12	13																				36173	12	38925	13	38546																				13	12	20	18	15																				29876	12	29792	13	30655																				13	12	12	12	12																				12	13	12	17	12																				12	34	12	18	27																																		
1:10:00	37.0																																		17965	12	19523	28	23058																				13	12	13	11	15																				33061	15	38821	12	47900																				13	14	13	12	13																				55340	12	56939	12	53781																				13	12	14	15	14																				49198	12	47712	12	46670																				13	20	15	12	13																				37910	12	40663	13	40342																				12	12	21	18	15																				32616	12	32604	13	33454																				12	12	12	12	13																				12	13	12	16	13																				12	33	12	18	27																																		
1:15:00	37.0																																		18203	11	19687	29	23273																				13	12	13	12	15																				33711	15	39428	12	48867																				13	15	13	12	13																				56760	12	58335	12	55103																				13	12	15	15	15																				50846	12	49238	12	48272																				13	20	15	12	13																				39365	12	42161	13	41795																				12	12	20	18	15																				35038	12	35182	13	35904																				12	12	12	12	12																				12	13	13	16	12																				12	33	12	18	27																																		
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1:25:00	37.0																																		18395	12	19993	29	23653																				12	12	13	12	15																				34247	14	40225	12	49874																				13	15	13	11	14																				58467	12	60365	12	56998																				13	11	15	15	14																				52852	12	51334	12	50423																				13	20	15	12	13																				41556	12	44459	13	43970																				13	12	21	18	16																				39477	12	39483	13	40479																				12	12	12	12	12																				12	13	13	17	12																				12	33	12	18	27																																		
1:30:00	37.0																																		18432	12	20082	29	23765																				13	12	13	12	15																				34260	15	40587	12	50383																				13	15	13	12	14																				59098	12	60935	12	57546																				13	12	14	16	14																				53605	12	52005	12	51146																				13	20	15	12	13																				42221	12	45101	13	44864																				13	12	21	18	16																				41483	12	41338	12	42460																				13	12	12	12	12																				11	12	12	16	12																				12	33	12	18	28																																		
1:35:00	36.9																																		18537	12	20118	28	23837																				13	11	13	12	15																				34668	14	40638	12	50486																				13	14	14	12	13																				59487	12	61296	12	58055																				13	12	15	15	14																				54112	12	52646	12	51712																				13	21	16	12	13																				42796	12	45912	13	45501																				13	12	21	18	15																				43210	13	42976	13	44169																				13	12	12	12	13																				12	13	12	16	12																				12	32	12	18	27																																		
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1:50:00	37.0																																		18594	12	20256	29	23925																				13	12	13	12	15																				34913	14	41193	12	50996																				13	15	14	12	13																				60233	12	62103	12	58522																				14	12	14	15	15																				54919	12	53449	12	52436																				13	25	15	12	13																				43829	12	46872	13	46492																				13	12	21	17	16																				47492	12	47338	13	48361																				13	12	12	13	12																				11	13	12	17	11																				12	32	12	18	27																																		
1:55:00	36.9																																		18601	12	20188	28	23913																				12	12	13	12	15																				34837	15	41174	12	50843																				13	15	13	12	13																				60141	12	61966	12	58765																				13	12	14	16	15																				55105	12	53479	11	52790																				13	25	16	11	13																				43973	12	47060	12	46685																				12	11	22	18	15																				48743	12	48578	13	49519																				12	12	12	12	12																				12	13	12	16	12																				12	32	13	17	27																																		
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2:15:00	37.0																																		18492	12	20165	28	23925																				12	12	13	12	15																				34849	14	41223	11	51067																				13	15	14	12	13																				60088	12	61905	12	58662																				14	12	15	15	14																				55120	12	53751	11	52665																				14	24	16	11	13																				44208	12	47251	13	46922																				13	12	21	18	16																				52284	12	52119	13	53238																				13	12	12	12	12																				11	13	12	16	12																				12	32	12	18	27																																		
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