

Software Version	3.16.10



Experiment File Path:	C:\Users\bnext\Documents\Biotek\experiments\20250708-202238-cytation5-pure-timecourse-gfp-pure-mg-sweep-iplus.xpt
Protocol File Path:	C:\Users\bnext\Documents\Biotek\protocols\cytation5-pure-timecourse-gfp.prt



Plate Number	Plate 1
Date	7/8/2025
Time	8:23:23 PM
Reader Type:	Cytation5
Reader Serial Number:	1705168
Reading Type	Reader

Procedure Details

Plate Type	Greiner 384 SV NoBind AutoMap
Well Selection	Runtime
Eject plate on completion	
Set Temperature	Setpoint 37C, Gradient 0 C
Start Kinetic	Runtime 6:00:00 (HH:MM:SS), Interval 0:05:00, 73 Reads
    Read	GFP-G70
	Fluorescence Endpoint
	Full Plate
	Filter Set 1
	    Excitation: 485/20,  Emission: 528/20
	    Optics: Top,  Gain: 70
	Light Source: Xenon Flash,  Lamp Energy: High,  Standard Dynamic Range
	Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
	Read Height: 10.5 mm
    Read	GFP-Gext
	Fluorescence Endpoint
	Full Plate
	Filter Set 1
	    Excitation: 485/20,  Emission: 528/20
	    Optics: Top,  Gain: extended
	Light Source: Xenon Flash,  Lamp Energy: High,  Extended Dynamic Range
	Read Speed: Normal,  Delay: 100 msec,  Measurements/Data Point: 10
	Read Height: 10.5 mm
End Kinetic	
Set Temperature	Incubator off

Layout
	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20	21	22	23	24
A																									Well ID
																									Name
B		SPL1		SPL2		SPL3		POS		NEG															Well ID
		I+ 8 mM		I+ 10 mM		I+ 12 mM		Positive		Negative															Name
C																									Well ID
																									Name
D		SPL1		SPL2		SPL3		POS		NEG															Well ID
		I+ 8 mM		I+ 10 mM		I+ 12 mM		Positive		Negative															Name
E																									Well ID
																									Name
F		SPL1		SPL2				POS		NEG															Well ID
		I+ 8 mM		I+ 10 mM				Positive		Negative															Name
G																									Well ID
																									Name
H																									Well ID
																									Name
I																									Well ID
																									Name
J																									Well ID
																									Name
K																									Well ID
																									Name
L																									Well ID
																									Name
M																									Well ID
																									Name
N																									Well ID
																									Name
O																									Well ID
																									Name
P																									Well ID
																									Name

GFP-G70:485,528

Time	T GFP-G70:485,528	B2	B4	B6	B8	B10	D2	D4	D6	D8	D10	F2	F4	F8	F10
0:00:00	32.5	30	25	31	24	34	35	22	23	39	33	27	31	33	24
0:05:00	37.0	29	27	23	29	29	27	29	13	31	38	27	29	36	14
0:10:00	37.0	25	23	24	31	32	23	20	21	29	29	25	21	19	19
0:15:00	37.0	28	22	29	31	28	29	24	23	31	26	23	23	33	21
0:20:00	37.0	17	27	28	38	26	26	25	20	36	27	23	36	43	26
0:25:00	37.1	23	21	20	47	26	29	25	27	55	31	22	23	55	19
0:30:00	37.0	22	25	26	99	25	33	28	26	100	34	22	34	110	17
0:35:00	37.0	43	27	24	247	38	21	32	29	244	34	26	32	262	25
0:40:00	37.0	34	32	31	556	31	33	38	31	558	37	34	39	578	15
0:45:00	37.0	35	41	34	1079	30	42	44	31	1063	34	30	40	1101	24
0:50:00	37.0	44	43	42	1859	36	45	46	39	1816	33	52	52	1880	22
0:55:00	37.0	56	49	47	2866	46	51	74	43	2790	32	57	61	2901	19
1:00:00	37.0	62	75	53	4035	40	69	73	59	4003	44	76	81	4026	26
1:05:00	37.0	87	84	62	5383	31	90	79	55	5268	33	87	91	5344	28
1:10:00	37.0	99	101	79	6851	48	100	116	76	6634	33	116	114	6639	19
1:15:00	37.0	138	128	98	8304	36	138	136	80	8023	39	138	147	8113	32
1:20:00	37.1	161	154	109	9701	36	180	170	101	9386	29	164	172	9374	32
1:25:00	37.1	189	189	128	11188	31	191	203	124	10779	34	197	203	10771	30
1:30:00	37.0	237	213	159	12509	37	229	246	114	12009	34	241	247	12005	34
1:35:00	37.1	287	251	172	13753	34	281	275	144	13184	35	292	278	13183	36
1:40:00	37.0	329	292	197	14818	50	329	320	156	14279	48	322	336	14182	39
1:45:00	37.0	375	333	219	16006	31	379	356	173	15381	33	381	372	15218	32
1:50:00	37.1	424	390	256	17023	38	433	413	196	16364	38	428	410	16174	24
1:55:00	37.0	468	429	274	17806	48	478	476	226	17271	43	479	457	17044	33
2:00:00	37.0	546	484	311	18798	49	533	522	245	18065	41	534	511	17806	40
2:05:00	37.0	599	528	345	19498	44	589	573	278	18762	37	603	558	18384	45
2:10:00	37.0	651	566	372	20281	39	649	617	288	19466	42	634	614	19002	41
2:15:00	37.0	718	614	403	20860	44	695	668	318	19979	40	696	654	19491	38
2:20:00	37.0	774	659	421	21490	47	760	723	345	20501	41	771	697	20066	44
2:25:00	37.0	821	713	463	22132	45	826	755	364	21130	38	802	742	20602	40
2:30:00	37.0	886	762	484	22441	45	861	819	388	21535	46	875	811	20937	46
2:35:00	37.0	919	799	526	22946	43	938	854	405	21965	36	913	842	21469	54
2:40:00	37.0	980	846	556	23389	50	967	912	439	22315	47	986	895	21711	39
2:45:00	37.0	1049	891	574	23669	43	1006	953	462	22707	49	1044	914	21896	36
2:50:00	37.0	1087	936	604	24006	51	1072	990	472	23065	44	1082	957	22466	47
2:55:00	37.0	1120	971	638	24283	42	1130	1035	502	23238	39	1124	998	22488	35
3:00:00	37.0	1177	1023	657	24589	50	1160	1076	516	23549	52	1154	1036	22772	55
3:05:00	37.0	1215	1037	683	24687	49	1205	1108	552	23678	53	1193	1096	22969	46
3:10:00	37.0	1266	1088	726	25093	44	1244	1156	568	23989	50	1235	1109	23109	46
3:15:00	37.0	1314	1122	743	25156	47	1270	1187	585	23975	59	1277	1146	23266	52
3:20:00	37.1	1345	1163	753	25503	52	1310	1225	614	24320	50	1313	1188	23583	51
3:25:00	37.0	1409	1201	787	25574	45	1344	1241	629	24446	60	1347	1211	23635	54
3:30:00	37.0	1430	1213	811	26008	56	1387	1287	645	24576	54	1404	1231	23763	48
3:35:00	37.0	1464	1240	821	26042	53	1421	1326	664	24862	51	1425	1260	23890	49
3:40:00	37.0	1480	1279	841	26070	55	1451	1345	698	25131	51	1444	1305	24037	53
3:45:00	37.0	1537	1305	872	26365	55	1466	1377	716	25097	56	1471	1326	24117	53
3:50:00	37.0	1548	1339	901	26280	60	1506	1395	712	25211	59	1522	1333	24192	53
3:55:00	37.0	1590	1354	920	26464	57	1544	1444	717	25207	59	1532	1375	24339	51
4:00:00	37.0	1604	1393	931	26639	62	1552	1474	766	25270	59	1582	1388	24368	66
4:05:00	37.0	1653	1412	956	26889	60	1589	1488	784	25413	62	1596	1419	24600	61
4:10:00	37.0	1680	1430	979	26785	63	1617	1514	802	25498	56	1615	1434	24547	66
4:15:00	37.0	1717	1461	982	27091	52	1649	1532	806	25655	59	1648	1472	24625	58
4:20:00	37.1	1745	1494	1017	26853	63	1674	1561	845	25549	60	1656	1491	24771	68
4:25:00	37.1	1748	1497	1013	26955	68	1704	1569	846	25833	63	1680	1492	24633	62
4:30:00	37.1	1771	1523	1043	27248	65	1733	1594	873	25808	63	1691	1524	25001	66
4:35:00	37.0	1796	1550	1045	27297	65	1722	1610	886	25770	63	1721	1537	25018	66
4:40:00	37.0	1799	1541	1069	27279	60	1745	1656	898	25976	67	1756	1541	24958	71
4:45:00	37.0	1831	1564	1096	27463	67	1756	1656	910	26070	68	1755	1571	24959	67
4:50:00	37.0	1866	1596	1107	27491	64	1790	1683	922	26061	63	1778	1593	25003	67
4:55:00	37.0	1871	1620	1116	27453	74	1816	1716	922	26108	66	1806	1607	25057	70
5:00:00	37.1	1894	1646	1127	27589	62	1826	1717	934	26064	76	1821	1626	25144	69
5:05:00	37.1	1897	1623	1146	27558	68	1827	1727	964	26136	74	1861	1608	25122	74
5:10:00	37.1	1912	1655	1159	27744	74	1865	1761	966	26361	79	1864	1659	25198	79
5:15:00	37.0	1964	1673	1162	27605	62	1891	1759	982	26273	80	1874	1657	25204	77
5:20:00	37.0	1964	1695	1187	27499	63	1902	1768	993	26228	71	1889	1681	25228	77
5:25:00	37.0	1977	1663	1203	27660	63	1914	1771	1007	26252	82	1898	1698	25125	76
5:30:00	37.0	1994	1710	1200	27765	72	1940	1815	1018	26394	76	1904	1707	25156	74
5:35:00	37.0	2015	1739	1218	27664	77	1930	1840	1020	26431	79	1915	1717	25165	74
5:40:00	37.0	2026	1735	1247	27781	73	1960	1837	1032	26461	75	1943	1734	25320	77
5:45:00	37.0	2036	1754	1246	27733	80	1973	1834	1063	26560	78	1928	1733	25400	90
5:50:00	37.1	2069	1775	1263	27973	85	1987	1846	1058	26588	78	1958	1765	25304	85
5:55:00	37.0	2061	1772	1249	27878	84	1991	1875	1077	26488	89	1972	1764	25452	78
6:00:00	37.0	2101	1783	1259	28102	86	2004	1881	1069	26541	76	1994	1792	25551	83

GFP-Gext:485,528

Time	T GFP-Gext:485,528	B2	B4	B6	B8	B10	D2	D4	D6	D8	D10	F2	F4	F8	F10
0:00:38	33.8	205	190	215	265	287	206	207	216	279	287	194	216	286	161
0:05:38	37.1	207	190	189	270	256	207	187	199	251	263	207	211	246	148
0:10:38	37.0	206	199	206	259	249	207	202	211	258	247	214	217	269	136
0:15:38	37.1	206	196	203	250	247	214	205	186	261	254	208	218	265	153
0:20:38	37.0	201	196	198	289	274	215	224	215	311	242	208	202	312	158
0:25:38	37.0	228	207	211	483	266	226	243	225	466	255	220	222	471	175
0:30:38	37.1	218	229	225	1034	268	230	245	208	1031	268	216	235	1137	156
0:35:38	37.1	240	224	240	2376	269	253	280	231	2406	279	251	281	2658	171
0:40:38	37.1	273	282	260	5217	279	273	284	252	5268	292	273	302	5626	177
0:45:38	37.0	312	311	281	10073	288	322	339	301	9945	264	329	366	10398	177
0:50:38	37.0	385	373	348	16815	287	377	399	319	16734	274	383	435	17155	177
0:55:38	37.0	456	450	403	25269	293	484	534	387	25188	305	520	509	25303	170
1:00:38	37.0	550	548	484	35202	287	600	650	427	34994	283	614	664	35851	197
1:05:38	37.0	731	731	576	46972	297	748	810	527	45890	310	762	833	46395	205
1:10:38	37.0	924	885	708	59147	312	955	978	592	57865	293	1006	1030	58340	279
1:15:38	37.0	1126	1092	831	71445	318	1188	1217	732	69268	302	1187	1260	69761	285
1:20:38	37.0	1427	1327	963	82903	311	1410	1534	837	80539	306	1437	1519	80788	276
1:25:38	37.0	1736	1606	1119	95060	304	1772	1763	929	92131	310	1823	1823	91505	281
1:30:38	37.0	1986	1880	1317	106304	314	2061	2085	1055	102772	306	2130	2146	102098	299
1:35:38	37.0	2412	2215	1519	116565	322	2428	2441	1242	112592	323	2493	2395	111567	300
1:40:38	37.0	2839	2541	1717	126446	308	2833	2777	1371	122342	336	2890	2807	121007	282
1:45:38	37.0	3237	2866	1920	135731	342	3185	3196	1567	130054	339	3288	3123	129357	308
1:50:38	37.0	3664	3274	2131	143670	332	3675	3593	1727	138706	337	3750	3595	137340	323
1:55:38	37.0	4100	3673	2403	152049	354	4170	3962	1912	145830	349	4205	3930	143652	326
2:00:38	37.0	4694	4064	2629	159673	360	4635	4461	2056	152465	333	4610	4420	150503	323
2:05:38	37.0	5086	4538	2889	164620	362	5065	4932	2272	158875	365	5074	4865	154857	335
2:10:38	37.1	5681	4842	3158	169524	342	5595	5274	2440	164804	328	5571	5262	160278	355
2:15:38	37.0	6086	5262	3458	175803	349	6029	5692	2692	169341	351	6046	5568	165421	331
2:20:38	37.0	6747	5720	3679	180372	368	6408	6127	2931	173649	381	6563	6031	169349	338
2:25:38	37.1	7095	6137	3944	186513	373	6946	6527	3129	178775	328	7033	6532	173888	374
2:30:38	37.0	7526	6504	4197	189253	354	7426	6936	3287	181925	386	7372	6704	176965	356
2:35:38	37.0	8001	6852	4503	193275	387	7893	7376	3487	184481	370	7888	7279	179799	380
2:40:38	37.0	8571	7202	4670	196338	362	8218	7709	3680	187369	376	8406	7536	182051	332
2:45:38	37.1	8885	7613	4937	199760	386	8641	8172	3876	189426	379	8767	7856	185142	356
2:50:38	37.0	9293	7887	5156	202271	382	9056	8349	4138	192591	405	9222	8344	187559	397
2:55:38	37.0	9819	8281	5422	204285	405	9455	8861	4284	195414	399	9532	8553	189144	392
3:00:38	37.1	10238	8765	5601	206934	413	9820	9230	4483	197843	417	9832	8881	191323	391
3:05:38	37.0	10477	8959	5836	209797	410	10110	9475	4688	199946	422	10291	9219	194251	397
3:10:38	37.1	10875	9128	6062	211081	417	10570	9831	4909	201078	401	10548	9408	195281	389
3:15:38	37.0	11299	9557	6314	213331	434	10973	10069	4968	202821	419	10908	9702	196943	412
3:20:38	37.0	11505	9925	6472	215125	426	11230	10446	5190	206759	423	11183	10128	198540	425
3:25:38	37.0	11973	10047	6706	215822	452	11535	10619	5425	205039	433	11516	10277	198712	435
3:30:38	37.0	11986	10398	6859	218271	443	11843	10965	5491	207940	443	11905	10524	200149	437
3:35:38	37.0	12427	10633	7029	218850	447	12077	11189	5694	207919	427	12078	10849	201887	431
3:40:38	37.0	12788	11009	7318	219901	434	12171	11576	5875	210717	463	12453	11058	202311	445
3:45:38	37.1	13192	11063	7439	220585	469	12646	11750	6136	211136	460	12686	11266	203582	465
3:50:38	37.0	13224	11392	7595	221999	475	12874	11896	6172	212095	469	12851	11474	204330	475
3:55:38	37.0	13489	11587	7826	221815	481	13029	12094	6284	211086	497	13235	11578	204804	477
4:00:38	37.0	13858	11637	7995	222772	485	13433	12477	6504	212794	497	13272	11988	204650	498
4:05:38	37.1	14154	12030	8056	223890	498	13635	12652	6661	213054	492	13645	12141	206668	518
4:10:38	37.1	14162	12353	8332	225483	511	13624	12885	6902	214609	499	13794	12310	206357	514
4:15:38	37.1	14573	12523	8404	226422	493	14000	12995	6807	215650	492	13848	12355	206662	558
4:20:38	37.0	14735	12653	8614	226004	497	14191	13290	7117	215466	513	14187	12506	208935	516
4:25:38	37.0	14712	12728	8805	227713	578	14427	13452	7115	216877	546	14194	12772	207448	536
4:30:38	37.0	14955	12991	8898	228498	561	14559	13476	7401	217321	533	14513	12987	208996	549
4:35:38	37.0	15241	13098	8990	228095	556	14685	13797	7515	216809	557	14660	13015	208837	553
4:40:38	37.0	15460	13343	9072	229747	558	14742	13835	7572	217640	578	14831	13238	210770	563
4:45:38	37.0	15584	13524	9300	229994	583	15072	14060	7750	218791	568	15089	13392	211063	587
4:50:38	37.0	15685	13414	9310	229391	584	15262	14451	7797	218399	572	15181	13397	209621	594
4:55:38	37.0	15920	13812	9488	230577	587	15408	14318	7836	218014	588	15277	13670	210925	584
5:00:38	36.9	16298	13837	9541	231812	574	15745	14575	7890	219193	595	15405	13769	210207	610
5:05:38	37.0	16224	14080	9655	230892	619	15684	14647	8081	219632	584	15645	13995	211620	599
5:10:38	37.0	16419	14217	9953	232479	585	15840	14754	8279	221225	610	15733	14172	212566	620
5:15:38	37.0	16476	14286	9922	232429	593	15909	14907	8395	220826	605	16013	14152	211842	656
5:20:38	37.1	16612	14502	10008	231898	629	16091	15110	8435	220891	624	15924	14196	211894	620
5:25:38	37.0	16737	14547	10304	231389	633	15990	15030	8566	220120	673	15940	14266	211679	623
5:30:38	37.0	16968	14594	10294	231928	627	16453	15356	8670	221563	666	16351	14573	212081	672
5:35:38	37.0	17131	14894	10279	232665	634	16536	15483	8694	221219	662	16291	14653	211756	691
5:40:38	37.0	17083	14858	10382	233320	637	16654	15640	8830	222170	689	16309	14621	213977	669
5:45:38	37.0	17437	14977	10521	234329	657	16715	15565	8948	222116	653	16717	14884	212494	708
5:50:38	37.0	17425	15120	10717	236188	656	16874	15810	9085	222850	644	16700	14935	214993	716
5:55:38	37.0	17588	15269	10627	234329	702	16864	15960	9064	222182	709	16848	14903	214213	722
6:00:38	37.0	17671	15325	10809	236357	706	17329	15980	9191	222904	712	17079	15081	214236	722

