LOCUS pOpen-deGFP 2812 bp DNA circular SYN 21-OCT-2025 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2812) AUTHORS . TITLE Direct Submission JOURNAL Exported Nov 13, 2025 from SnapGene 8.2.1 https://www.snapgene.com FEATURES Location/Qualifiers source 1..2812 /mol_type="other DNA" /organism="synthetic DNA construct" CDS complement(33..49) /codon_start=1 /label=lacZ-alpha (fragment) /note="pLannotate" /note="/database=snapgene" /note="/fragment=True" /note="/identity=100.0" /note="/match_length=9.8" /note="/other=CDS" /translation="TGRRF" terminator 50..81 /label=tonB terminator /note="pLannotate" /note="/database=snapgene" /note="/fragment=False" /note="/identity=96.9" /note="/match_length=100.0" /note="/other=terminator" misc_feature 98..101 /label=MC1-TU-L misc_feature 102..912 /label=pT7-UTR1-deGFP-t7hyb6 /label=pT7-tetO-UTR1-plamGFP-t7hyb6 primer_bind 102..121 /label=pT7 misc_feature 122..149 /label=UTR1 misc_feature 150..157 /label=MC0U-SP /label=CDS Prefix misc_feature 150..155 /label=BsaI_Bind CDS 158..835 /codon_start=1 /label=deGFP /translation="MELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICT TGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKT RAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKI RHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTA AGI" misc_feature 836..853 /label=CDS Suffix regulatory 854..892 /label=T7hyb6 Terminator misc_feature 893..912 /label=Terminator Spacer terminator 950..997 /label=T7 terminator /note="pLannotate" /note="/database=snapgene" /note="/fragment=False" /note="/identity=100.0" /note="/match_length=100.0" /note="/other=terminator" rep_origin 1085..1673 /label=ori /note="pLannotate" /note="/database=snapgene" /note="/fragment=False" /note="/identity=99.7" /note="/match_length=100.0" /note="/other=rep_origin" CDS complement(1864..2724) /codon_start=1 /label=AmpR /note="pLannotate" /note="/database=snapgene" /note="/fragment=False" /note="/identity=99.7" /note="/match_length=100.0" /note="/other=CDS" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(join(2725..2812,1..17)) /label=AmpR promoter /note="pLannotate" /note="/database=snapgene" /note="/fragment=False" /note="/identity=100.0" /note="/match_length=100.0" /note="/other=promoter" ORIGIN 1 caaatagggg ttccgcgcac ctgcaccagt cagtaaaacg acggccagta gtcaaaagcc 61 tacgaccgga ggcttttgac ttggttcagg tggagtggga gtaatacgac tcactatagg 121 gaataatttt gtttaacttt aagaaggagg gtctctaatg gagcttttca ctggcgttgt 181 tcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 241 gggcgagggc gacgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 301 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 361 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 421 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 481 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 541 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 601 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 661 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 721 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 781 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tctgatgaag 841 agcttagaga ccgagataac agatacttcg gtatctgtta tctgtttttt ttctaagcat 901 cacactgccg tccgctgagg tgtcaatcgt cggagccgct gagcaataac tagcataacc 961 ccttggggcc tctaaacggg tcttgagggg ttttttgcat ggtcatagct gtttcctgag 1021 agcttggcag gtgatgacac acattaacaa atttcgtgag gagtctccag aagaatgcca 1081 ttaatttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1141 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1201 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1261 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1321 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1381 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1441 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1501 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 1561 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1621 gtggtttttt tgtttgcaag cagcagatta cacgcagaaa aaaaggatct caagaaggcc 1681 tactattagc aacaacgatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 1741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 1801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 1861 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 1921 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 1981 ccccagtgct gcaatgatac cgcgagaacc acgctcaccg gctccagatt tatcagcaat 2041 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 2101 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 2161 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 2221 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 2281 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 2341 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 2401 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 2461 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 2521 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 2581 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 2641 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 2701 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 2761 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aa //