LOCUS       Linear-T7-toehol        1203 bp    DNA     linear   UNA 29-JUN-2026
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Bnext Bio
  TITLE     Direct Submission
  JOURNAL   Exported Jun 29, 2026 from SnapGene 8.2.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /organism="unspecified"
     source          join(1..18,19..30)
                     /mol_type="genomic DNA"
                     /note="This feature has 2 segments:
                        1:  1 .. 18 / #ffffff
                        2: 19 .. 30 / #ffffff"
                     /organism="unspecified"
     source          1171..1182
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /organism="unspecified"
     source          1183..1203
                     /mol_type="genomic DNA"
                     /note="color: #ffffff"
                     /organism="unspecified"
     primer_bind     1..18
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    19..24
                     /label=BbsI
                     /note="color: #a6acb3"
     misc_feature    27..30
                     /label=MC1-TU-L
                     /note="color: #a6acb3"
     misc_feature    31..49
                     /label=T7 promoter
                     /note="color: #faac61"
     misc_feature    50..130
                     /label=toehold 9
                     /note="color: #c7b0e3"
     misc_feature    131..1093
                     /label=PLA1 (codon-optimized to E. coli)
                     /note="phospholipase A1 (PLA1) from Serratia sp. MK1"
                     /note="color: #b4abac"
     CDS             131..1093
                     /codon_start=1
                     /label=PLA1
                     /note="color: #b4abac"
                     /translation="MSMSLSFTSAIAPAAIQPPMVRTQPEPLSSSQPVEASATKAPVAT
                     LSQNSLNAQSLLNTLVSEISAAAPAAANQGVTRGQQPQKGDYTLALLAKDVYSTGSQGV
                     EGFNRLSADALLGAGIDPASLQDAASGFQAGIYTDNQQYVLAFAGTNDMRDWLSNVRQA
                     TGYDDVQYNQAVSLAKSAKAAFGDALVIAGHSLGGGLAATAALATGTVAVTFNAAGVSD
                     YTLNRMGIDPAAAKQDAQAGGIRRYSEQYDMLTGTQESTSLIPDAIGHKITLANNDTLS
                     GIDDWRPSKHLDRSLTAHGIDKVISSMAEQKPWEAMANA"
     regulatory      1112..1150
                     /label=T7hyb6 Terminator
                     /note="color: #ffffff"
     misc_feature    1151..1170
                     /label=Terminator Spacer
                     /note="color: #a6acb3"
     misc_feature    1171..1174
                     /label=MC1-TU-R
                     /note="color: #a6acb3"
     misc_feature    complement(1177..1182)
                     /label=BbsI
                     /note="color: #a6acb3; direction: LEFT"
     primer_bind     complement(1183..1203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
ORIGIN
        1 gtaaaacgac ggccagtgga agacatggag taatacgact cactataggg ttgtgtggta
       61 ttgtcttgtt tgcatacaga aacagaggag atatgcaatg aagacaataa acctgaaagg
      121 agcgcaaaag atgtctatgt cactgtcttt tacatcggct atcgcgcccg ctgctattca
      181 accgccaatg gtccgtacac agccggaacc gttgtcctct agtcagcctg tggaagcgag
      241 cgctacaaaa gcccctgtcg caaccctgag tcagaactcc ctcaatgccc aatcattgct
      301 caataccctc gtctccgaaa tctctgccgc cgctccggcg gcagctaatc aaggagtcac
      361 gcgcgggcag cagccacaga aaggagacta tactttggcg ctgctcgcta aagatgttta
      421 ttcaaccggt tcacagggtg tcgaagggtt caaccgcctg tcagctgatg cgttgttagg
      481 agcaggtata gatccggcaa gtctgcagga cgccgcttcc ggctttcaag cagggattta
      541 tacggataac cagcagtacg ttcttgcttt tgctgggacc aatgatatgc gagattggtt
      601 gagcaacgtc cgtcaagcga ccggatacga cgatgtacag tataatcagg ctgtcagctt
      661 ggcaaaatct gctaaagccg ccttcggcga cgcgcttgtt attgccggcc atagcttagg
      721 gggcggcctc gccgcaacgg ctgcattggc tacagggact gtggctgtta cttttaacgc
      781 agctggggtg tcagattata cgcttaatcg tatgggaatt gaccctgcgg cagccaaaca
      841 ggatgcccag gccggtggga tccgtcgcta tagcgaacaa tacgatatgc ttactgggac
      901 acaagaatca acaagcctca tcccagacgc tattggtcat aaaattacct tagcgaacaa
      961 cgatacattg agtggcattg atgactggcg tccctcaaaa cacctggatc gttcactgac
     1021 tgcacatggt atcgataaag ttatctcgag tatggcggaa cagaagccgt gggaagcaat
     1081 ggccaacgca tgatgaagag cttagagacc gagataacag atacttcggt atctgttatc
     1141 tgtttttttt ctaagcatca cactgccgtc cgctatgtct tccatggtca tagctgtttc
     1201 ctg
//